安装R bioconductor基因组软件包
问题描述:
我一直在尝试使用称为seqplots的工具来分析一些测序数据。该工具贯穿生物导体,需要安装基因组软件包。该Bioconductor的网站给出了如下的安装说明:安装R bioconductor基因组软件包
要安装这个包,启动R和输入:
source("https://bioconductor.org/biocLite.R")
biocLite("BSgenome.Dmelanogaster.UCSC.dm6")`
如果我在R终端输入这个我得到以下几点:
BioC_mirror: https://bioconductor.org
Using Bioconductor 3.2 (BiocInstaller 1.20.3), R 3.2.4 Revised (2016-03-16
r70336).
Installing package(s) ‘BSgenome.Dmelanogaster.UCSC.dm6’
installing the source package ‘BSgenome.Dmelanogaster.UCSC.dm6’
trying URL 'https://bioconductor.org/packages/3.2/data/annotation/src/contrib/BSgenome.Dmelanogaster.UCSC.dm6_1.4.1.tar.gz'
Content type 'application/x-gzip' length 34147677 bytes (32.6 MB)
downloaded 32.6 MB
'\\icnas4.cc.ic.ac.uk\gaughey'
CMD.EXE was started with the above path as the current directory.
UNC paths are not supported. Defaulting to Windows directory.
* installing *source* package 'BSgenome.Dmelanogaster.UCSC.dm6' ...
** R
** inst
** preparing package for lazy loading
** help
*** installing help indices
** building package indices
** testing if installed package can be loaded
*** arch - i386
Warning in library(pkg_name, lib.loc = lib, character.only = TRUE, logical.return = TRUE) :
there is no package called 'BSgenome.Dmelanogaster.UCSC.dm6'
Error: loading failed
Execution halted
*** arch - x64
Warning in library(pkg_name, lib.loc = lib, character.only = TRUE, logical.return = TRUE) :
there is no package called 'BSgenome.Dmelanogaster.UCSC.dm6'
Error: loading failed
Execution halted
ERROR: loading failed for 'i386', 'x64'
* removing '\\icnas4.cc.ic.ac.uk/gaughey/R/win-library/3.2/BSgenome.Dmelanogaster.UCSC.dm6'
The downloaded source packages are in
‘C:\Users\gaughey\AppData\Local\Temp\Rtmpcdmz8E\downloaded_packages’`
我已经尝试安装其他具有类似结果的基因组,并且无法解决此问题。 任何人都可以帮忙吗?我很可能忽略了一些简单的东西 - 我是一个R(或者任何编程)的完全新手。我将不胜感激任何帮助。
谢谢, 加布里埃尔
答
我建议您在尝试biocLite(pkgs = "BSgenome.Hsapiens.NCBI.GRCh38", dependencies = TRUE)
之前尝试运行biocLite("BSgenome")
和library(BSgenome)
(它存储给定生物体的全基因组序列)。
尝试更新您的R版本和bioconductor。我可以毫无问题地安装它,而且我有'使用Bioconductor 3.3(BiocInstaller 1.22.3),R 3.3.1(2016-06-21).'而您有'使用Bioconductor 3.2(BiocInstaller 1.20.3), R 3.2.4修订'。 – Vandenman